Epithelial Cell Junctions

Overview

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Snapshot

  • A 40-year-old woman presents with a pruritic rash of 2 weeks duration. She reports a persistent rash over her extremities with very itchy blisters that do not break easily. On physical exam, she has multiple 1-5 cm tense bullae on her abdomen and arms. Skin biopsy with direct immunofluorescence shows linear pattern of IgG and C3 deposits along the epidermal-dermal junction. (Bullous pemphigoid)

Tight Junction

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  • Tight junction
    • also known as zonula occludens
    • location
      • between adjacent epithelial cells
    • composition
      • claudins
      • occludins
    • function
      • diffusion barrier between adjacent cells
      • prevents paracellular movement of solutes
  • maintains physiochemical separation of tissue compartments

Adherens Junction

  • Adherens junction
    • also known as belt desmosome, zonula adherens, and intermediate junctions
    • location
      • between adjacent epithelial cells
    • composition
      • e-cadherin
    • function
      • forms a belt that connects actin cytoskeletons to plasma membrane
      • actin filaments connect with E-cadherin
        • cadherins are Ca2+-dependent adhesion proteins
  • loss of E-cadherins promote metastasis

Desmosome

  • Desmosome
    • also known as spot desmosome and macula adherens
    • location
      • between adjacent epithelial cells
    • composition
      • desmoplakin
      • desmoglein 
        • connects keratinocytes in stratum spinosum
      • desmocollin
    • function
      • structural support
      • couples intermediate filament to plasma membrane in adjacent cells
    • diseases
      • pemphigus vulgaris 
  • autoantibodies target desmoglein component of desmosomes

Gap Junction

  • Gap junction 
    • location
      • between adjacent epithelial cells
    • composition
      • connexons with central channel 
    • function
      • forms channel for electrical and chemical communication between adjacent cells
  • permits exchange of ions, regulatory molecules, and small metabolites through pores

Hemidesmosome

  • Hemidesmosome
    • location
      • between epithelial cell and basement membrane
    • composition
      • integrin
    • function
      • connects epithelial cells to underlying basement membrane
      • integrins bind to laminin-5 and type IV collagen, attaching epithelial cell to extracellular matrix
    • diseases
      • bullous pemphigoid 
        • autoantibodies target hemidesmosome